转基因产品检测技术及其差异比较

本文介绍了常用的转基因产品检测方法,包括针对核酸的聚合酶链式反应(PCR)和针对蛋白质的酶联免疫吸附法(ELISA),并对几种检测方法的优缺点进行比较。     

Risks to the aquatic ecosystem from the application of Metarhizium anisopliae for locust control in Australia

Laboratory tests of Metarhizium anisopliae var acridum Driver & Milner, at a dose of 1.3 x 10(6) conidia ml-1, had no adverse effects on nymphs of mayfly, Ulmerophlebia sp or 8-week-old fry of the rainbow fish, Melanotaenia duboulayi Castelnau. This dose was toxic to the cladoceran, Ceriodaphnia dubia Richard, causing 100% mortality in 48 h. When this test was repeated at doses of up to 6.7 x 10(3) conidia ml-1, there was only 5% mortality after 192 h. Spraying of artificial water sources with a very high dose of the fungus as an aqueous spray resulted in 80-130 conidia ml-1 at 15 cm depth in the first 24 h after spraying. The conidia rapidly settled out and were absent from the top 15 cm layer of water after about 50 h. A similar experiment using the oil formulation as used in field control resulted in a 2- to 20-fold lower level of conidia in the water. Finally, sampling actual water sources in spray areas revealed a very low level of contamination of the water, with a maximum mean level of 29 conidia ml-1 in the first 24 h after treatment. Thus the level of conidia likely to enter water during control campaigns is a small fraction of that required to kill cladocerans, the only sensitive non-target organism tested. It is concluded that the biopesticide is very unlikely to pose any hazard to aquatic organisms.     

Helicobacter infection in dogs and cats: facts and fiction

The discovery of the spiral bacterium Helicobacter pylori and its causative role in gastric disease in humans has brought a dramatic change to gastroenterology. Although spiral bacteria have been known for more than a century to infect the stomachs of dogs and cats, recent research has been conducted mainly in the wake of interest in H. pylori. H. pylori has not been found in dogs and only very rarely in cats and zoonotic risk is minimal. A variety of other Helicobacter spp. can infect the stomach of pets; however, their pathogenic role is far from clear, and they have a small but real zoonotic potential. The prevalence of gastric Helicobacter spp. in dogs and cats is high, irrespective of clinical signs, and as in human medicine, mode of transmission is unclear. The relationship of Helicobacter spp. to gastric inflammation in cats and dogs is unresolved, with inflammation, glandular degeneration, and lymphoid follicle hyperplasia accompanying infection in some but not all subjects. Circulating anti-Helicobacter immunoglobulin G antibodies have been detected in 80% of dogs with naturally acquired infection and most dogs and cats with experimental infection. The gastric secretory axis is similar in infected and uninfected cats and dogs and no relationship of infection to gastrointestinal ulcers has been found. Differences in the pathogenicity of Helicobacter spp. are apparent, because infection with H pylori is associated with a more severe gastritis than infection with other Helicobacter spp. in both cats and dogs. Rapid urease test, histopathology, and touch cytology are all highly accurate invasive diagnostic tests for gastric Helicobacter-like organisms in dogs and cats, whereas culture and polymerase chain reaction are the only means to identify them to the species level. Urea breath and blood tests or serology can be used to diagnose Helicobacter spp. noninvasively in dogs and cats. Most therapeutic studies in pets have not shown long-term eradication of Helicobacter spp. Whether this is due to reinfection or recrudescence has not been established.     

The effect of the herbicide diuron on soil microbial activity

The inhibitory effect of the herbicide diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] on microbial activity in red Latosol soil was followed using microcalorimetry. The activity of the micro-organisms in 1.50 g of soil sample was stimulated by addition of 6.0 mg of glucose and 6.0 mg of ammonium sulfate under 35% controlled humidity at 298.15 (+/- 0.02) K. This activity was determined by power-time curves that were recorded for increasing amounts of diuron, varying from zero to 333.33 micrograms g-1 soil. An increase in the amount of diuron in soil caused a decrease of the original thermal effect, to reach a null value above 333.33 micrograms g-1 of herbicide. The power-time curve showed that the lag-phase period and peak time increased with added herbicide. The decrease of the thermal effect evolved by micro-organisms and the increase of the lag-phase period are associated with the death of microbial populations caused by diuron, which strongly affects soil microbial communities.     

A method to test wheat leaves for their reactions to inoculation with Septoria species

The construction and application is described of a polystyrol humidity box in which wheat leaves, while continuing to function as parts of living plants, can be tested for their reactions toSeptoria spp. in an atmosphere nearly saturated with water, as is required for successful infection. The method is simple, accurate ans inexpensive.Samenvatting Voor dit doel is een z.g. vochtdoos geconstrueerd (Fig. 1A). Het te toetsen blad wordt daar doorheen geleid en in de doos geïnoculeerd met een druppel conidiën-suspensie van de schimmel. Onder het blad staat wat water in de doos (Fig. 1B). Na afsluiting ontstaat in de doos de hoge luchtvochtigheid van bijna 100% die nodig is voor de infectie. Dit wordt op deze wijze eenvoudig en goedkoop gerealiseerd. De bladeren die zo worden getoetst blijven onbeschadigd functioneren aan de plant. Na enkele dagen kan de doos worden geopend en kan de symptoom-ontwikkeling worden afgewacht en gevolgd (Fig. 3A, 3B en 4). De methode leent zich voor nauwkeurig werk en vereist zeer weinig infectiemateriaal. De Technische en Fysische Dienst voor de Landbouw (TFDL), Wageningen, ontwierp en construeerde een statief voor het gebruik van de vochtdozen in serie (Fig. 2).     

水中爆破对海洋生物及海洋环境的影响研究

本文主要根据１９８３年６月在莱州湾现场模拟水中爆破勘探石油对水产资源的影响实验，分别实验了爆破所产生的声压波对鱼、虾、蟹、贝类和浮游生物的影响，采集并分析了爆炸前后的海水样品。由实验结果得出，水中爆破对海水水质的影响主要因素是：悬浮物、无机氮等。水中爆破所产生的声压波对海洋生物有一定的致死作用，但致死范围不大，而对水产资源的幼体和浮游生物伤害较大。     

Populations, Community Composition and Biomass of Aquatic Organisms in the Floodwater of Rice Fields and Effects of Field Management

As rice fields are managed for improved rice production, many studies on aquatic organisms in the floodwater have been restricted to agronomically important members such as blue-green algae (BGA) and Azolla , and ecological studies on common aquatic organisms were relatively limited to specific group members. In the present paper, studies on all the aquatic organisms in the floodwater, from protozoa to insects and fishes, were reviewed worldwide from the viewpoint of rice field ecology, paying attention to their populations, seasonal variations, antagonistic relations and biomass, and the effect of field management. Most studies conducted in India and in the Southeast Asian countries dealt with the BGA and green algal populations. In contrast, protozoa and aquatic animals were extensively studied in rice fields in Europe. Japanese studies in the past were intermediate between the previously cited studies. They generally described specific groups depending on the authors' interests, and surprisingly few studies dealt with the community level and prey-predator relationships. Field management such as fertilizer and pesticide application, water management and plowing was found to play a major role in determining the populations of aquatic organisms. Overview of research on aquatic organisms in the floodwater of rice fields suggests several directions for future studies.     

管材对给水管附生生物膜微生物分布的影响

结合微生物学传统培养和分子生物学现代技术,分析了无规聚丙烯(Po lypropy lene R andom,PPR)管、镀锌钢管和铜管3种室内给水管附生生物膜微生物群落。结果表明,在以上3种管道上发育1年的附生生物膜中,分别有6种、5种和3种优势异养菌被分离出来;α变形杆菌纲优势异养菌所占比例在PPR管、镀锌钢管和铜管附生生物膜中依次增加,而β变形杆菌纲和放线菌纲优势异养菌所占比例则依次减小;镀锌钢管和PPR管附生生物膜中均存在作为优势异养菌的机会病原体,而铜管附生生物膜中则未分离出作为优势异养菌的机会病原体。     

海底大型垃圾对海洋生物的影响及其潜在生态效应

海底大型垃圾是海洋垃圾的重要组成部分,但目前关于海洋垃圾的研究主要集中于漂浮垃圾,而针对海底大型垃圾的研究较少。海底大型垃圾通常不易分解和迁移,这使其不仅能直接改变海洋环境和底质,还能通过影响海洋生物生存与生长进而威胁海洋生态系统健康。本文从定殖、缠绕、围困和摄食等4个角度系统地总结了目前海底大型垃圾对海洋生物的影响及其潜在生态效应的研究现状及进展,并对未来研究方向进行了展望。本文旨在从生物角度客观、全面地综述海底大型垃圾对底栖海洋生态系统的影响,以期为评估海底大型垃圾对海洋生物多样性的作用机制,及海底大型垃圾综合管控与治理提供科学依据。     

竖壁贴附送风改善冷藏库内流场特性

果蔬贮藏期间,冷藏库内的气流分布是影响果蔬贮藏品质和送风能量利用率的关键因素,合理的气流组织对冷藏库内流场分布是至关重要的。该研究以某苹果冷藏库为研究对象,采用竖壁贴附送风方式,并对其流场建立了三维稳态的SST湍流模型来研究库内流场的分布特性以及货物的冷却效果,同时与传统的冷风机直吹送风的流场特性进行了对比。结果表明：在相同送风量下,相比冷风机直吹送风方式,竖壁贴附送风使得库内温度不均匀系数和速度不均匀系数分别降低了31%和47%。而且竖壁贴附送风使库内空气与货物的换热更加充分,进而使得送风的能量利用率提高了19%。因此,竖壁贴附送风方式应用于冷藏库是一种有效且节能的气流组织方式。